A Drop-in Replacement for LR(1) Table-Driven Parsing

This paper presents a construction method for a deterministic one-symbol look-ahead LR parser which allows non-terminals in the parser look-ahead. This effectively relaxes the requirement of parsing the reverse of the right-most derivation of a string/sentence. This is achieved by replacing the deterministic push down automata of LR parsing by a two-stack automata. The class of grammars accepted by the two-stack parser properly contains the LR(k) grammars. Since the modification to the table-driven LR parsing process is relatively minor and mostly impacts the creation of the goto and action tables, a parser modified to adopt the two-stack process should be comparable in size and performance to LR parsers.</p

Genetics and livestock breeding in the UK: Co-constructing technologies and heterogeneous biosocial collectivities

Cattle and sheep breeders in the UK and elsewhere are increasingly being encouraged to use a variety of genetic technologies to help them make breeding decisions. The technology of particular interest here is ‘classical’ statistical genetics, which use a series of measurements taken from animals’ bodies to provide an estimate of their ‘genetic merit’ known as Estimated Breeding Values (EBVs). Drawing on empirical research with the representatives of national cattle breed societies and individual cattle breeders the paper explores the complex ways in which they are engaging with genetic breeding technologies. The concept of ‘heterogeneous biosocial collectivity’ is mobilised to inform an understanding of processes of co-construction of breeding technologies, livestock animals and humans. The paper presents case studies of livestock breeding collectivities at different scales, arguing that the ways in which the ‘life’ of livestock animals is problematised is specific to different scales, and varies too between different collectivities at the same scale. This conceptualisation problematises earlier models of innovation-adoption that view farmers as either ‘adopters’ or ‘non-adopters’ of technologies and in which individual attitudes alone are seen as determining the decision to adopt or not adopt. Instead, the paper emphasises the particularity and specificity of co-construction, and that the co-construction of collectivities and technologies is always in process

Single tube liquid biopsy for advanced non-small cell lung cancer

The need for a liquid biopsy in non-small cell lung cancer (NSCLC) patients is rapidly increasing. We studied the relation between overall survival (OS) and the presence of four cancer biomarkers from a single blood draw in advanced NSCLC patients: EpCAM(high) circulating tumor cells (CTC), EpCAM(low) CTC, tumor-derived extracellular vesicles (tdEV) and cell-free circulating tumor DNA (ctDNA). EpCAM(high) CTC were detected with CellSearch, tdEV in the CellSearch images and EpCAM(low) CTC with filtration after CellSearch. ctDNA was isolated from plasma and mutations present in the primary tumor were tracked with deep sequencing methods. In 97 patients, 21% had >= 2 EpCAM(high) CTC, 15% had >= 2 EpCAM(low) CTC, 27% had >= 18 tdEV and 19% had ctDNA with >= 10% mutant allele frequency. Either one of these four biomarkers could be detected in 45% of the patients and all biomarkers were present in 2%. In 11 out of 16 patients (69%) mutations were detected in the ctDNA. Two or more unfavorable biomarkers were associated with poor OS. The presence of EpCAM(high) CTC and elevated levels of tdEV and ctDNA was associated with a poor OS; however, the presence of EpCAM(low) CTC was not. This single tube approach enables simultaneous analysis of multiple biomarkers to explore their potential as a liquid biopsy

Professional Competencies in Computing Education

Competency-based learning has been a successful pedagogical approach for centuries, but only recently has it gained traction within computing. Competencies, as defined in Computing Curricula 2020, comprise knowledge, skills, and professional dispositions. Building on recent developments in competency and computing education, this working group examined relevant pedagogical theories, investigates various skill frameworks, reviewed competencies and standard practices in other professional disciplines such as medicine and law. It also investigated the integrative nature of content knowledge, skills, and professional dispositions in defining professional competencies in computing education. In addition, the group explored appropriate pedagogies and competency assessment approaches. It also developed guidelines for evaluating student achievement against relevant professional competency frameworks and explores partnering with employers to offer students genuine professional experience. Finally, possible challenges and opportunities in moving from traditional knowledge-based to competency-based education were also examined. This report makes recommendations to inspire educators of future computing professionals and smooth students' transition from academia to employment

High-resolution analysis of HLA class I alterations in colorectal cancer

BACKGROUND: Previous studies indicate that alterations in Human Leukocyte Antigen (HLA) class I expression are frequent in colorectal tumors. This would suggest serious limitations for immunotherapy-based strategies involving T-cell recognition. Distinct patterns of HLA surface expression might conceal different immune escape mechanisms employed by the tumors and are worth further study. METHOD: We applied four-color multiparameter flow cytometry (FCM), using a large panel of alloantigen-specific anti-HLA-A and -B monoclonal antibodies, to study membranous expression of individual HLA alleles in freshly isolated colorectal cancer cell suspensions from 21 patients. RESULTS: Alterations in HLA class I phenotype were observed in 8 (38%) of the 21 tumors and comprised loss of a single A or B alleles in 4 cases, and loss of all four A and B alleles in the other 4 cases. Seven of these 8 tumors were located on the right side of the colon, and those showing loss of both HLA-A and -B membranous expression were all of the MSI-H phenotype. CONCLUSION: FCM allows the discrimination of complex phenotypes related to the expression of HLA class I. The different patterns of HLA class I expression might underlie different tumor behavior and influence the success rate of immunotherapy